ABSTRACT
Emblic medicine is a popular natural source in the world due to its outstanding healthcare and therapeutic functions. Our preliminary results indicated that the quality of emblic medicines might have an apparent regional variation. A rapid and effective geographical traceability system has not been designed yet. To trace the geographical origins so that their quality can be controlled, an integrated spectroscopic strategy including spectral pretreatment, outlier diagnosis, feature selection, data fusion, and machine learning algorithm was proposed. A featured data matrix (245 × 220) was successfully generated, and a carefully adjusted RF machine learning algorithm was utilized to develop the geographical traceability model. The results demonstrate that the proposed strategy is effective and can be generalized. Sensitivity (SEN), specificity (SPE) and accuracy (ACC) of 97.65％, 99.85％ and 97.63％ for the calibrated set, as well as 100.00％ predictive efficiency, were obtained using this spectroscopic analysis strategy. Our study has created an integrated analysis process for multiple spectral data, which can achieve a rapid, nondestructive and green quality detection for emblic medicines originating from seventeen geographical origins.
ABSTRACT
This study was aimed to establish and optimize the reaction system of MSAP for analysis on DNA methylation inCyathula officinalis Kuan of Sichuanmedicinal. The tender leaves ofC. officinalisKuan were used as materials. Orthogonal method was used in the study of main factors which affected the system quality of MSAP. The results showed that the optimal reaction systems of MSAP included: enzyme digestion (20μL), 300 ng DNA, 1.5μL EcoRI, 1.5μL HpaII or 1.5μL MspI (HpaII); ligation (25μL): 15μL digestion products, 1μL EcoR I adaptor, 1μL Hpa II/Msp I adaptor, 0.2μL T4 ligase; pre-amplication mixture (25μL): ligation products 1μL, rTaq polymerase 1 U, each primer 1.5μL, dNTP 2μL; selective amplification mixture (25μL): pre-amplification product was diluted 50 times, rTaq polymerase 1 U, each primer 1.5μL, dNTP 3μL. The optimal MSAP reaction system was used to screen for 6 pairs of effective primers from 256 pairs of primers of MSAP. It was concluded that the optimized system ensured the stable and clear bands and the screened 6 pairs of primers were with good specificity. It provided useful references for further studies of epigenetic onC. officinalisKuan DNA methylation and MSAP analysis for other medicinal plants.
ABSTRACT
This article is aimed to establish the method of quality standards for highland barley Monascus.Microscopic and TLC were adopted to identify Araliataibaiensis. Referring the relevant method in Chinese Pharmacopoeia (2010 edition) for the determination of its moisture, total ash, acid insoluble ash and extracts,lovastatin in acid and lactone form was determined using high performance liquid chromatography. The characteristics of highland barley Monascus in microscopic and TLC were obvious and specific. The contents in barley red yeast are: water 6.04%-8.78%, total ash 2.15%-2.92%, acid insoluble ash 0.23%-0.29%, water extracts 27.99%-30.17%; alcohol extracts 14.12%-18.51%. The ranges of lovastatin in acid and lactone form are 0.11%-0.29% and 0.02%-0.08%, respectively. The established method is simple, accurate, reliable and duplicable, and can be used to control the quality of highland barley Monascus.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the history of the medicinal uses, resources, distribution, habitat and population characteristic of Coptis deltoidea, and provide basis for the protection and rational development and utilization of Radix Coptidis Deltoideae.</p><p><b>METHOD</b>The relevant literature and data was scrutinized and herbarium was compared, interview and field survey methods were carried out.</p><p><b>RESULT</b>The medicinal history, resources, distribution, population characteristic and protective strategy of C. deltoidea were summarized. The sustainable development of C. deltoidea was discussed.</p><p><b>CONCLUSION</b>The resource is endangered, the germplasm resources should be intentionally protected and ensure the sustainable development and utilization of C. deltoidea.</p>
Subject(s)
Conservation of Natural Resources , Methods , Coptis , Drugs, Chinese Herbal , Metabolism , Ecosystem , Endangered SpeciesABSTRACT
<p><b>OBJECTIVE</b>To analyze the content of tanshinone IIA in Radix Salviae Miltiorrhizae from different habitats are regularly altered and find out the deviation between the studies.</p><p><b>METHOD</b>The data of the content of tanshinone IIA in samples of Radix Salviae Miltiorrhizae from different areas and related information since 1997 was collected and statistically analyzed.</p><p><b>RESULT AND CONCLUSION</b>The content of tanshinone IIA in wild Salvia was generally higher than that in the cultivated Salvia, and the content in nearly half cultivated Salvia was less than 0.2%. There existed a big difference between the used statistical model, and the data related the content in samples from Sichuan was the most divisive. The coefficient of variation of wild Salvia was below the cultivated Salvia, and the cultivated Salvia from different areas showed different coefficients of variation. The content of tanshinone IIA was lower when samples were extracted using the heating reflux method.</p>
Subject(s)
Abietanes , Phenanthrenes , Salvia miltiorrhiza , ChemistryABSTRACT
OBJECTIVE:To analyze the effects of environmental factors (soil,climate,etc.) on the quality of Radix Polygalae.METHODS:The contents of polygalic acid were determined by HPLC.Climate factors including altitude,average annual temperature,average temperature of July,annual precipitation,annual sunshine hours and non-frost days were collected through on-site inspection or from the local meteorological data. Water content,pH and nutritional elements of the soils were determined by routine method,and the reletionship between the content of polygalic acid and the factors were analyzed.RESULTS:There was linear relationship between the content of polygalic acid and climate factors such as average temperature of July,annual precipitation,annual sunshine hours and non-frost days.There was also linear relationship between polygalic acid and soil factors such as total nitrogen,total phosphor,available phosphor,available kalium,organic matters and water content. CONCLUSION:The main climate factors that affect polygalic acid content were annual sunshine hours and non-frost days,while the main soil factors affecting the content of polygalic acid were the content of available phosphor and soil water content.